546-pBad24-sfGFP: Difference between revisions
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Latest revision as of 15:53, 17 February 2012
Cloning of 546-pBad24-sfGFP
[edit]Purpose: Cloning of 546-pBad24-sfGFP by genesynthesis
Date: 2012-2-7--
Notebook:003732
Page:
Clone ID:546
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-2-7
PCR template is Ecoli-sfGFP1 to Ecoli-sfGFP20
Primer-F Ecoli-sfGFP1
Primer-R Ecoli-sfGFP20
| band 3 |
band 4 | |
| PCR product |
pBad24 545 | |
| digest enzymes |
EcoR1 + HindIII | |
Colony Screening
[edit]Date: 2012-2-17
Make miniprep and digest with EcoR1 + HindIII
clone 1,2,3,5,6 may be right ,need to sequence
Result intepretation/Summary
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Conclusion
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