Lentivirus QC 2-24-2012: Difference between revisions
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Latest revision as of 15:43, 27 February 2012
Notebook: Xiaojing Wang
Page: 004184,004185
493 pLVXPlus2-App-IRES-mCherry
493 lentivirus production-293T in plates
Use psPAX2 (503) 15 ug + VSVG (505) 7.5 ug + DNA 22.5 ug in each plate
The Gene Expresso Max of 493 lentivirus production is Lot:DSP01042012.
2 days after transfection(2-24-2012), take out 0.5 ml medium and add to 1 well of 293T cells growing on 12 well plate (50% confuluency). Add 1000X polybrene, so the final concentration is 1X .
Incubate for 3 days, take pictures.
