1029-pHisGST: Difference between revisions
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Latest revision as of 13:58, 26 March 2012
Cloning of pHisGST (1029)
Purpose: to clone pHisGST
Date: 2012-3-21 to 2012-3-23
Notebook: Xiaojing Wang
Page: 004203
Clone ID: 1029
Experimenters: Xiaojing Wang
Results
digest pGST 455 with Nde1 + EcoR1, digest pET28b with Nde1 + EcoR1.
ligate, transform top10
Select 4 clones to mini-prep
1029 plasmids are digested by Nde1 + EcoR1
clone 1029-1,-2,-3,-4 may be correct.