531-pLVXPlus3-IRES-mCherry

Cloning of pLVXPlus3-IRES-mCherry (531)
Purpose: to clone pLVXPlus3-IRES-mCherry
Date: 2012-3-27 to 2012-3-30
Notebook: Xiaojing Wang
Page: 004170,004210
Clone ID: 531
Experimenters: Xiaojing Wang
Results

1 Annealed oligos:

pLVXPlus3-F

pLVXPlus3-R

2 Digest pLVXPlus2-IRES-mCherry (174) with EcoR1 + Xho1, cut out vector.

Ligation, Transformation

Select 5 clones to mini-prep
531 plasmids are digested by BamH1 + Mlu1 or Xba1 + Mlu1

531-1,-5 may be correct.

Select 531-1 to sequence.