Adenovirus Production 293F: Difference between revisions
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Latest revision as of 16:44, 14 April 2012
1. Culture 293F cells in 293 SFM II medium in suspension.
2. Add MOI 0.1TU/cell virus to 3 X 10^5 cells in 60 ml medium.
3. Harvest cells at day 8 after infection. Yield should be 1X10^11 TU