1060-pLVX-CAG-MCS-IRES-mCherry

Cloning of pLVX-CAG-MCS-IRES-mCherry(1060)
Purpose: to clone pLVX-CAG-MCS-IRES-mCherry
Date: 2012-4-2 to 2012-4-4
Notebook: Xiaojing Wang
Page: 004211,004215
Clone ID: 1060
Experimenters: Xiaojing Wang
Results

Digest pCAGMKOSiE, 313(13#) with Nde1 + EcoR1, digest pLVXPlus3-IRES-mCherry,531 with Nde1 + EcoR1.

Band 1 is 313 digested by Nde1.

Band 2 is 313 digested by EcoR1.

Band 3 is 313 digested by Nde1+EcoR1.

Ligate, transform STBL3,A

Select 5 clones to mini-prep
1060 plasmids are digested by Nde1 + EcoR1

Clone 1060-4,-5 may be correct.

Select 1060-4 to sequence.