1129 pDB.His.PfuMBP

May 2, 2012

PCR using template from gene synthesis

digest with Kpn1 + Nde1, clone into Kpn1+ Nde1 digested 360 pDB.His.MBP

Select 6 clones to mini-prep
1129 plasmids are digested by Nde1 + Kpn1

Select 1129-5 to sequence.

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Cloning of 1129 pDB.His.PfuMBP

Purpose: Cloning of 1129 pDB.His.PfuMBP

Date: 2012-5-17---

Notebook:0

Page:

Clone ID:1129

Experimenters: yingying

Results

cloning

	band 1	band 2
	PCR product	360 pDB.His.MBP
enzymes	Kpn1 + Nde1

2012-5-18

	band 3	band 4
	PCR product	360 pDB.His.MBP
enzymes	Kpn1 + Nde1

Colony Screening

Date: 2012-5-21

Make miniprep and digest with Kpn1 + Nde1

clone 1,2,3 need to sequence

Result intepretation/Summary

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Conclusion

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