1192 pCDNA3.2: Difference between revisions
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Latest revision as of 14:31, 31 May 2012
5/25/2012
digest pCDNA3.1 with Nhe1 + Xba1
ligate to annealed pCDNA3.1-T + pCDNA3.1-B
5' CTAGC GGATCC GAATTC ggtacc AAGCTT TTAATTAA Ctcgag GCGGCCGC GATATC T 3'
3' g CCTAGG CTTAAG ccatgg TTCGAA AATTAATT Gagctc CGCCGGCG CTATAG AGATCT 5'
pCDNA3.1-T
CTAGCGGATCCGAATTCggtaccAAGCTTTTAATTAACtcgagGCGGCCGCGATATCT
pCDNA3.1-B
TCTAGAGATATCGCGGCCGCctcgaGTTAATTAAAAGCTTggtaccGAATTCGGATCCg
transform DH5a
5/27/2012
digest miniprep with PacI + Nde1
5/31/2012