1204 pET28b-pigIFN-alpha: Difference between revisions
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Latest revision as of 14:24, 7 June 2012
Cloning of 1204 pig IFN alpha
[edit]Purpose: Cloning of pig IFN alpha
Date: 2012-5-29
Notebook:
Page:
Clone ID: 1204
Experimenters: Paul
Results
[edit]cloning
[edit]Date: 5-29-2012
PCR using Kapa HiFi.
6/2/2012
cut out band and purify using gel extraction kit. digest with Nde1 + Xho1. ligate into Nde1 + Xho1 digested pET28b.
transform DH5alpha
6/7/12
digest miniprep with Nde1 + Xho1
Colony Screening
[edit]Date:
Result intepretation/Summary
[edit]