1247-pCDNA3.1-PBasic-PH: Difference between revisions
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Latest revision as of 14:46, 19 July 2012
Cloning of pCDNA3.1-PBasic-PH(1247)
Purpose: to clone pCDNA3.1-PBasic-PH
Date: 2012-7-13 to 2012-7-19
Notebook: Xiaojing Wang
Page: 005296
Clone ID: 1247
Experimenters: Xiaojing Wang
Results
synthesize pBasic + PH
Digest with Xho1 + BamH1, ligate to Xho1 + BamH1 digested pCDNA3.1, 1226
Transform DH5alpha
Pick up 6 clones to mini-prep
1247 plasmids are digested by Xho1 + BamH1
Clone 1247-1,-2,-3,-4,-5,-6 may be correct.
Pick up 1247-2 to sequence.