1164 pFUSE-aKOD-H-CHIg-hG1: Difference between revisions
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Latest revision as of 09:58, 26 July 2012
Cloning of 1164 pFUSE-aKOD-H-CHIg-hG1
[edit]Purpose: Cloning of 1164 pFUSE-aKOD-H-CHIg-hG1
Date: 6/1/2012
Notebook: 0
Page:
Clone ID: 1164
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: 6/1/2012
Synthesized template by gene synthesis, cut out band for cloning next week
Date: July 19, 2012
Digested DNA (lane 3) and pFUSE-CHIg-hG1,1161 (lane 4) with restriction enzymes EcoR1 + Nhe1
Cut out bands in lanes 3 and 4. Saved in box and ligate together tomorrow
Colony Screening
[edit]Date: July 25, 2012
Expected size ~500 bp, all samples are positive for insert
Result intepretation/Summary
[edit]