1121 pET28b-Exo-1: Difference between revisions
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Latest revision as of 15:07, 27 July 2012
Cloning of 1121 pET28b-Exo-1
[edit]Purpose: Cloning of 1121 pET28b-Exo-1
Date: July 23, 2012
Notebook:
Page:
Clone ID: 1121
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: July 24, 2012
See lane 2, expected size 1428 bp
PCR from MG1665 genomic DNA
Exo-I-Nde1-F
Nde1
cagacaCAT ATGATGAATGACGGTAAGCAAC
Exo-I-Xho1-R
(GGCAGTACGCGGAAGAGATTGTC CTCGAG cagatc)
gatctgCTCGAGGACAATCTCTTCCGCGTACTGCC
Cut out band, purify, digest with RE Ndel and Xho1, with vector pET28b
See lanes 6 (insert) and 7 (vector), cut out bands, purify and ligate together and transform
Colony Screening
[edit]Date: July 27, 2012
Digested MiniPrep with RE Nde1 and Xho1, see lanes 13-18
All samples are positive for insert, submitted sample 2 for sequencing
Result intepretation/Summary
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Conclusion
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