1268 pTXB1-RNaseA: Difference between revisions
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Latest revision as of 14:25, 30 July 2012
Cloning of 1268 pTXB1-RNaseA
[edit]Purpose: Cloning of 1268 pTXB1-RNaseA by gene synthesis
Date: July 26, 2012
Notebook: 0
Page:
Clone ID: 1268
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: July 26, 2012
See lane 1, amplified DNA sequence by gene synthesis (25 cycles, HotStartPhusion), expected size ~400 bp
Cut out band, purify and digest with 1254 vector with RE Nde1 and Xho1
See lane 1 (insert) and lane 2 (vector), cut out purify and ligate together. Transform
Colony Screening
[edit]Date: July 30, 2012
Digested MiniPrep with RE Nde1 and Xho1
Expected size ~400 bp
Samples 3 and 5 (lanes 3 and 5 respectively) may be positive for insert
Result intepretation/Summary
[edit]