1273 pTXB1-RnaseH: Difference between revisions
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Latest revision as of 15:50, 6 August 2012
Cloning of 1273 pTXB1-RnaseH
[edit]Purpose: Cloning of 1273 pTXB1-RnaseH
Date: July 30, 2012
Notebook:
Page:
Clone ID: 1273
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: July 30, 2012
See lane 1
PCR using genomic DNA from K-12 substr. MG1655
RnaseH-Nde1-F AGCTCTCATatgcttaaacaggtagaaattttc
RnaseH-Xho1-R gtctcgctcgagaacttcaacttggtagcctg
Clone into Nde1 + Xho1 digested 1254 pTXB1-PT-ELK
Expected size 496 bp
Cut out band and save in box
Date: August 1, 2012
Digested insert (lane 1) with RE Nde1 + Xho1, vector is not good, digest again tomorrow, join with lane 1, purify and ligate together
Colony Screening
[edit]Date: August 6, 2012
Digested MiniPrep with RE Nde1 + Xho1, expected size 496 bp
Sample 1-5 may be correct
Send sample 3 to sequencing
Result intepretation/Summary
[edit]
Conclusion
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