1299 pET-6H-VPMLK-NLS: Difference between revisions
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Latest revision as of 15:57, 15 August 2012
Cloning of 1299 pET-6H-VPMLK-NLS
[edit]Purpose: Cloning of 1299 pET-6H-VPMLK-NLS
Date: August 13, 2012
Notebook:
Page:
Clone ID: 1299
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 13, 2012
Ligate annealed oligos to Nco1 + Nde1 digested 1128 pDB.His.PfuMBP-(tev) vector, see lane 1
His-VPMLK-NLS-T
cATGGCACATCATCATCACCATCATGTTCCGATGCTGAAACCGAAAAAAAAACGCAAAGTGca
cGTGTAGTAGTAGTGGTAGTACAAGGCTACGACTTTGGCTTTTTTTTTGCGTTTCACgtat
His-VPMLK-NLS-B
tatgCACTTTGCGTTTTTTTTTCGGTTTCAGCATCGGAACATGATGGTGATGATGATGTG
Transform
Date: August 15, 2012
Ligate annealed oligos to Nco1 + Nde1 digested pDB.His.MBP, V14 vector, see lane 1
His-VPMLK-NLS-T
cATGGCACATCATCATCACCATCATGTTCCGATGCTGAAACCGAAAAAAAAACGCAAAGTGca
cGTGTAGTAGTAGTGGTAGTACAAGGCTACGACTTTGGCTTTTTTTTTGCGTTTCACgtat
His-VPMLK-NLS-B
tatgCACTTTGCGTTTTTTTTTCGGTTTCAGCATCGGAACATGATGGTGATGATGATGTG
Transform
Colony Screening
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Result intepretation/Summary
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Conclusion
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