1301 pET-6H-VPTLK-NLS: Difference between revisions
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Latest revision as of 14:30, 17 August 2012
Cloning of 1301 pET-6H-VPTLK-NLS
[edit]Purpose: Cloning of 1301 pET-6H-VPTLK-NLS
Date: August 13, 2012
Notebook:
Page:
Clone ID: 1301
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 13, 2012
Ligate annealed oligos with Nco1 + Nde1 digested 1128 pDB.His.PfuMBP-(tev), see lane 3
His-VPTLK-NLS-T
cATGGCACATCATCATCACCATCATGTTCCGACCCTGAAACCGAAAAAAAAACGTAAAGTGca
cGTGTAGTAGTAGTGGTAGTACAAGGCTGGGACTTTGGCTTTTTTTTTGCATTTCACgtat
His-VPTLK-NLS-B
tatgCACTTTACGTTTTTTTTTCGGTTTCAGGGTCGGAACATGATGGTGATGATGATGTGc
Tranform
Date: August 15, 2012
Ligate annealed oligos with Nco1 + Nde1 digested pDB.His.MBP, V14, see lane 3
His-VPTLK-NLS-T
cATGGCACATCATCATCACCATCATGTTCCGACCCTGAAACCGAAAAAAAAACGTAAAGTGca
cGTGTAGTAGTAGTGGTAGTACAAGGCTGGGACTTTGGCTTTTTTTTTGCATTTCACgtat
His-VPTLK-NLS-B
tatgCACTTTACGTTTTTTTTTCGGTTTCAGGGTCGGAACATGATGGTGATGATGATGTGc
Transform
Colony Screening
[edit]Date: August 17, 2012
Digested MiniPrep with RE Nde1 and Nco1
Very faint inserts at expected oligo size ~180 bp
Result intepretation/Summary
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Conclusion
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