1207-pDB.His.MBP-(3C)

Cloning of 1207-pDB.His.MBP-(3C)

Purpose: Cloning of 1207-pDB.His.MBP-(3C)

Date:  2012-6-29----

Notebook:

Page:

Clone ID: 1207

Experimenters:yingying lu

Cloning

lane 1: digest 360 pDB.His.MBP with Nde1 + BamH1,
ligate to annealled oligos
MBP-3C-T
T Agc CTG GAA GTT CTG TTC CAG GGG CCC CAT ATG CTG CAG gct agc g
MBP-3C-B
gatccgctagcCTGCAGCATATGGGGCCCCTGGAACAGAACTTCCAGgc

Date: August 17, 2012

lane 1: digest 360 pDB.His.MBP with Nde1 + BamH1,
ligate to annealled oligos
MBP-3C-T
T Agc CTG GAA GTT CTG TTC CAG GGG CCC CAT ATG CTG CAG gct agc g
MBP-3C-B
gatccgctagcCTGCAGCATATGGGGCCCCTGGAACAGAACTTCCAGgc

Colony Screening

Date: August 20, 2012

Digest with Pst + Xba1--->1.2 kb

Clones 1, 2, 3, 4, 5 and 6 may be correct

Result intepretation/Summary

<br>

Conclusion

<br>