PKA ELISA 4-5-2011

Notebook zhen tao #10

Page 2092

plate was coated with 2ug/ml PKA-C-alpha in TBS; 100 ul/well; then blocked by 1% BSA in TBS and 10% glycerol. each sample was diluted 1/10000 with blocking buffer.

samples	OD450-1	OD450-2	OD450-3	average
control	0.025	0.021	0.019	0.022
31	0.056	0.056	0.056	0.056
32	0.078	0.080	0.080	0.079
447673	0.110	0.122	0.155	0.129
447674	0.054	0.059	0.056	0.056
positive control	0.569	0.557	0.556	0.561

--Ztao 15:35, 5 April 2011 (EST)