PCMV5-Fc-(3c)-NPR1-Cyto-WPRE-oriP: Difference between revisions
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Latest revision as of 13:59, 20 September 2012
Cloning of 1377pCMV5-Fc-(3c)-NPR1-Cyto-WPRE-oriP
[edit]Purpose: subcloning of 3C into pDB.His.MBP(V14)
<br> Date: 2012-09-18<br>
<br> Notebook:&nbsp;
Page:
Clone ID: 1375
Experimenters: Xiuli
Results
[edit]Cloning
09-19-2012 A.Restriction enzyme digestion: Vector V14 pDB.His.MBP and 1197 pDB.His.GST.3C with NdeI and XhoI, B.Gel purification (elute with 15 ul H2O), and ligation with T7 ligase at RT for2 hs (total volume in 30ul) C Transformation: DH5a + 20 ul ligation Re.