PKA ELISA 4-7-2011

Notebook Zhen Tao #10

Page 2097~2098

all samples were diluted 1/2000 with blocking solution;
Control: only 2nd antibody (anti-human) was used;
sample 31~40: normal people serum;
sample 447667~447669: prostate cancer people serum;
sample 447670~447672: colon cancer people serum;
sample 447673~447674: breast cancer people serum;
positive: anti-PKA-C-alpha was used (1/10000)

OD450 data for normal and cancer samples are corrected by substrating the average of control (0.018).

samples	OD450-1	OD450-2	OD450-3	average of normal serum	cancer/normal
control	0.017	0.018	0.020	0.018
31	0.107	0.098	0.109	0.124
32	0.169	0.157	0.165
33	0.144	0.133	0.152
34	0.092	0.126	0.071
35	0.154	0.204	0.159
39	0.122	0.130	0.098
40	0.065	0.087	0.077
447667	0.499	0.650	0.463		4.317
447668	0.387	0.492	0.346		3.280
447669	0.170	0.257	0.117		1.455
447670	0.232	0.246	0.188		1.782
447671	0.077	0.082	0.080		0.638
447672	0.405	0.433	0.410		3.342
447673	0.274	0.281	0.258		2.176
447674	0.095	0.095	0.101		0.777
postitive	0.964	0.976	0.989		7.847

--Ztao 13:29, 7 April 2011 (EST)