1293pET30b-2Tat-6Arg: Difference between revisions

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Latest revision as of 13:39, 14 November 2012

Cloning of 1293pET30b-2Tat-6Arg

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Purpose: Cloning of 1293pET30b-2Tat-6Arg

Date: Nov 1, 2012

Notebook: 

Page:

Clone ID: 1293

Experimenters: Shanyi

Results

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Cloning

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Date: Nov 1, 2012


Synthesized template via gene synthesize, expected size ~147 bp


Digested insert  and  vector  with  Nde1 + Xho1



Ligate , transform 

digest with NdeI and XhoI


Colony Screening

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Result intepretation/Summary

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Conclusion

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