1517 pT7A3-MBP-(3C)-sfGFP: Difference between revisions

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Latest revision as of 14:48, 21 December 2012

Cloning of 1517 pT7A3-MBP-(3C)-sfGFP

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Purpose: Cloning of 1517 pT7A3-MBP-(3C)-sfGFP

Date:Dec, 2012

Notebook: 

Page:

Clone ID: 1517

Experimenters: shanyi

Results

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Cloning

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PCR sfGFP from 1005 pBad24-Tat-sfGF



digest with Nhe1 + Not1, clone into Nhe1 + Not1 digested 1514 pT7A3-MBP-(3C)

Colony Screening

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digestion using Sal1 + HindIII

Result intepretation/Summary

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Conclusion

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