42-pLVX-EF1-MCS-Puro: Difference between revisions

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Latest revision as of 13:33, 14 January 2013

Cloning of 42-pLVX-EF1-MCS-Puro

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Purpose: Cloning of 42-pLVX-EF1-MCS-Puro

Date: 2013-1-11---2-13-1-14

Notebook:0

Page:

Clone ID:42

Experimenters: yingying

Results

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subcloning

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cut 534 pLVX-EF1-IRES-EGFP to release EF1 promoter  to 164 pLVX-Plus2 with Nde1 + EcoR1

Colony Screening

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Date: 2013-1-14

Make miniprep and digest with Nde1 + EcoR1

Result intepretation/Summary

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Conclusion

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