1608-pT7A3-K8XHis-MBP-(3C): Difference between revisions

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Latest revision as of 14:41, 16 January 2013

Cloning of 1608-pT7A3-K8XHis-MBP-(3C)

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Purpose: Cloning of 1608-pT7A3-K8XHis-MBP-(3C)

Date: 2013-1-10---2013-1-16

Notebook:0

Page:

Clone ID:1608

Experimenters: yingying

Results

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cloning

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lane 1.Digest PCR product with BspH1 + Nde1,

lane 2.clone into1279pT7A3 vector's Nco1 + Nde1 sites.



Colony Screening

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Date: 2013-1-16

Make miniprep and digest with Xba1 + Nde1

Result intepretation/Summary

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Conclusion

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