562 pEC-EcoR1: Difference between revisions
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Latest revision as of 14:01, 20 May 2013
Cloning of 562 pEC-EcoR1
[edit]Purpose: Cloning of 562 pEC-EcoR1
Date: 2013-4---
Notebook:0
Page:
Clone ID:562
Experimenters: Shanyi
Results
[edit]cloning
[edit]gene synthesis
join two fragments by PCR
digest with Nde1+Xho1
Colony Screening
[edit]Date: 2013-4-
Make miniprep and digest with Nde1+Xho1
cloning
[edit]FIX ONE GAP,USE 562-6 as template,use EcoRI-1-1and EcoRI-1-6, EcoRI-1-7 and EcoRI-2-12 as primers
join two fragments by PCR
digest with Nde1+Xho1
Colony Screening
[edit]Date: 2013-4-
Make miniprep and digest with Nde1+Xho1
