1763 pT7A30-Tks-sso7d: Difference between revisions

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Latest revision as of 15:22, 21 June 2013

Cloning of 1763 pT7A30-Tks-sso7d

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Purpose: Cloning of 1763 pT7A30-Tks-sso7d

Date: June 10, 2013

Notebook: 

Page:

Clone ID: 1763

Experimenters: Shanyi

Results

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Cloning

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subclone into 1734 pT7A30-sso7d-KX using Nde1 + Kpn1 from 1265 pEC-Tks, run on 0.8% DNA gel

digest1734 with Nde1 + Kpn1,digest 1265 with DraIII + AlwN1+Nde1 + Kpn1


ligate and transform NEB Turbo


Colony screen

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digest with Nde1 + Kpn1 



Result intepretation/Summary

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Conclusion

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