1759 pCold-6H-(TEV)-MBP-(3C)-Keap1: Difference between revisions
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Latest revision as of 13:27, 17 July 2013
Cloning of 1759 pCold-6H-(TEV)-MBP-(3C)-Keap1
[edit]Purpose: Cloning of Keap1 into pCold-6H-(TEV)-MBP-(3C)
Date: June 11, 2013
Notebook: ???
Page: ???
Clone ID: 1759
Experimenters: Shanyi
Results
[edit]Cloning
[edit]subclone KEAP1 from 1676 pCold-MBP-(3C)-KEAP1 to 1756 pCold-6H-(TEV)-MBP-(3C) using Nde1 + Xho1
Colony screen
[edit]digest with Nde1 +Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]protein expression
[edit]
| lane |
clone |
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| 1 to 8 |
NRF2 |
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| 9 |
Cre |
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| 10 |
1759,pCold-6H-(TEV)-MBP-(3C)-Keap1 |
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| 11 |
1759,pCold-6H-(TEV)-MBP-(3C)-Keap1 |