1799-pCold-6His-MCS-(3C)-MBP: Difference between revisions

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Latest revision as of 13:45, 31 July 2013

Cloning of 1799-pCold-6His-MCS-(3C)-MBP

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Purpose: Cloning of 1799-pCold-6His-MCS-(3C)-MBP

Date: 2013-7-29---2013-7-31

Notebook:0

Page:

Clone ID:1799

Experimenters: yingying

Results

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cloning

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lane
 3
vector
 1798 pCold-6His-MCS-(TEV-3C)-MBP
enzymes
 Nde1 + Xho1

ligate to annealed oligos Nde1-MCS-Xho1-T2 + Nde1-MCS-Xho1-B2.


Colony Screening

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Date: 2013-7-31

Make miniprep and digest with Sal1 + BglII

clone1,2,8 may be right, need to sequence



Result intepretation/Summary

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Conclusion

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