User contributions for Paul.xu

31 January 2011

• 18:4618:46, 31 January 2011 diff hist +1,840 VP16-expression 2 No edit summary current
• 18:2518:25, 31 January 2011 diff hist 0 N File:MBP vp16 purification2.jpg No edit summary current
• 18:2518:25, 31 January 2011 diff hist 0 N File:Mbp vp16 purification.jpg No edit summary current

30 January 2011

• 15:4715:47, 30 January 2011 diff hist 0 N File:Lentivirus packaging mix optimizationg 3 day2.JPG No edit summary current
• 11:4211:42, 30 January 2011 diff hist 0 N File:Packagingmix optimization 1 day3 post infection.JPG No edit summary current
• 11:4211:42, 30 January 2011 diff hist 0 N File:Packagingmix optimization 2 day1 post infection.JPG No edit summary current
• 11:4211:42, 30 January 2011 diff hist 0 N File:Packagingmix optimization 3 day2 after transfection.JPG No edit summary current

29 January 2011

• 13:5213:52, 29 January 2011 diff hist 0 N File:Packagingmix optimization 1 infection day1.JPG No edit summary current
• 13:4713:47, 29 January 2011 diff hist 0 N File:Packagingmix optimization 2 day2.JPG No edit summary current

28 January 2011

• 18:2818:28, 28 January 2011 diff hist 0 N File:Vp16 MBP purification.jpg No edit summary current
• 17:2617:26, 28 January 2011 diff hist 0 N File:Lentivirus packaging mix optimizationg 2 day1.JPG No edit summary current

27 January 2011

• 09:3609:36, 27 January 2011 diff hist 0 N File:Lentivirus packaging mix optimization day 2.JPG No edit summary current

26 January 2011

• 17:4217:42, 26 January 2011 diff hist 0 N File:Lentivirus vector transfection optimization.JPG No edit summary current

11 January 2011

• 19:4719:47, 11 January 2011 diff hist +3,108 N Lv master mix Created page with "Day 2 Image:Lv_mastermix_optimazation.JPG    pSMP-EGFP       Packaging mix       Transfection 1&nbs..." current
• 19:3919:39, 11 January 2011 diff hist +65 N File:Lv mastermix optimazation.JPG Lv mastermix optimization and comparison of transfection reagents current

12 December 2010

• 15:3015:30, 12 December 2010 diff hist +3,893 VP16-expression No edit summary current
• 13:3413:34, 12 December 2010 diff hist +23 File:VP16 purification WB.jpg uploaded a new version of "File:VP16 purification WB.jpg": 6X-His-VP16 expression and purification current

19 November 2010

• 19:4819:48, 19 November 2010 diff hist +23 N File:NusA VP16 TEV Digestion.jpg NusA_VP16_TEV_Digestion current
• 19:4119:41, 19 November 2010 diff hist +42 N File:NusA VP16 TEV Digestion 2.jpg NusA-VP16 fusion protein digested with TEV current

14 November 2010

• 14:5314:53, 14 November 2010 diff hist +339 N Kythera Biopharma AKR1C4 Created page with "Aldo-keto reductase family 1 member C4: AKR1C4 <br> Transform STBL3 with plasmid DNA pLenti-HA-AKR1C4.29, grow on LB/Amp plate. <br> pick 3 clones, grow in LB/AMP, make minipre..." current
• 14:5014:50, 14 November 2010 diff hist +41 N File:PLenti-HA AKR1C4 BamH1 Xho1.jpg pLenti-HA-AKR1C4 digest with BamH1 + Xho1 current

13 November 2010

• 12:5212:52, 13 November 2010 diff hist +43 N File:Vp16 NusA MBP GST expression.jpg expression of VP16 fusion to NusA, MBP, GST current
• 12:4712:47, 13 November 2010 diff hist +29 N VP16-MBP-expression moved VP16-MBP-expression to VP16-expression current

8 November 2010

• 18:1718:17, 8 November 2010 diff hist +56 N File:VP16 pDB His GST pDB His NusA Nde1 Xho1.JPG pDB.His.GST-Vp16 & pDB.His.NusA, digest with Nde1 + Xho1 current

5 November 2010

• 17:3917:39, 5 November 2010 diff hist +85 N File:POTB7-PEDF-R-EcoR1 Xho1.JPG pOTB7-PEDFR cut with EcoR1 & Xho1, NM_020376, size should be 1.5 kb, cut only ~600 bp current
• 16:4416:44, 5 November 2010 diff hist +1,459 N Aldolase-Expression Created page with "400x400px|Aldolase Vp16 MBP fusion expression.jpg Expression of MBP-Aldolase and MBP-VP16 in BL21 (DE3)<br> MW: 10(bottom), ..." current
• 16:3416:34, 5 November 2010 diff hist +33 N File:Vp16-MBP expression.jpg VP16-MBP Expression in BL21 cells current
• 16:2116:21, 5 November 2010 diff hist +69 N File:Aldolase Vp16 MBP fusion expression.jpg expression of Aldolase-MBP and VP16-MBP fusion proteins in BL21(DE3). current
• 12:4712:47, 5 November 2010 diff hist +33 N Category:Baculovirus Created page with "This is the Baculovirus category." current
• 10:3110:31, 5 November 2010 diff hist +32 Category:Lentivirus No edit summary current
• 10:3010:30, 5 November 2010 diff hist +32 Category:Adenovirus No edit summary current
• 10:3010:30, 5 November 2010 diff hist +25 N Category:AAV Created page with "This is the AAV category." current
• 10:3010:30, 5 November 2010 diff hist +42 N Category:Protein Purification Created page with "This is the Protein Purification category." current
• 10:3010:30, 5 November 2010 diff hist +40 Category:Protein Expression No edit summary current
• 10:2910:29, 5 November 2010 diff hist +32 N Category:Sequencing Created page with "This is the Sequencing category." current
• 10:2910:29, 5 November 2010 diff hist +28 N Category:Clones Created page with "This is the Clones category." current
• 10:2910:29, 5 November 2010 diff hist +29 Category:Vectors No edit summary current

31 October 2010

• 19:5419:54, 31 October 2010 diff hist +72 N File:VP16 pET28b Clone24 Nde1 Xho1 NEB Fastdigest.JPG VP16-pET28b (Clone24) digest with Nde1+Xho1 (Fastdigest left, NEB right) current
• 19:5319:53, 31 October 2010 diff hist +60 N File:VP16 pET28b pDBHisMBP Nde1 Xho1 NEB.JPG VP16-pET28b,VP16-pDBHisMBP digest with Nde+Xho1 (NEB enzyme) current
• 16:5716:57, 31 October 2010 diff hist +51 N File:VP16 pET28b pDBHisMBP Nco Xho1.JPG VP16-pET28b, VP16-pDBHisMBP, digest with Nco1, Xho1 current
• 15:4215:42, 31 October 2010 diff hist +51 N File:VP16 pET28b pDBHisMBP Nde1 Xho1.JPG Vp16-pET28b, VP16-pDB.His.MBP digest with Nde1+Xho1 current

29 October 2010

• 12:4012:40, 29 October 2010 diff hist +29 N File:10 28 2010 VP16.tar.gz.txt VP16-pET28b sequencing result current

27 October 2010

• 13:1413:14, 27 October 2010 diff hist +21 N File:Vp16 pET28 Nde1 Xho1.jpg Vp16-pET28b Nde1+Xho1 current

26 October 2010

• 17:5317:53, 26 October 2010 diff hist +42 N File:Vp16-pET28b PCR Screening 2.jpg VP16-pET28b PCR Screening 2nd exp, 2nd gel current
• 17:4017:40, 26 October 2010 diff hist +37 N File:VP16 pET28b PCR Screening 2.jpg PCR screening of VP16-pET28b colonies current

25 October 2010

• 23:0523:05, 25 October 2010 diff hist +59 N File:VP16 Xho Xba.JPG Vp16-pET28b digested with Xho1, or Xho1+Xba1, or undigested current
• 22:4722:47, 25 October 2010 diff hist +26 N File:PVp16-1.txt Vp16 gene synthesis design current
• 18:5618:56, 25 October 2010 diff hist +91 N File:IMG 0159.JPG Digest sample 1, 2 and 8 with Nde1, Xho1 (Fermantas) and AseI (NEB) using Fermentas buffer. current
• 18:0418:04, 25 October 2010 diff hist +365 N Template:Cloning Created page with "<noinclude> This is the "Cloning" template. It should be called in the following format: <pre> {{Cloning |Title= |Purpose= |Date= |Scientist= }} </pre> Edit the page to see the t..." current
• 17:3017:30, 25 October 2010 diff hist +27 N File:IMG 0158.JPG Nde1+ Xho1 cut, NEB enzymes current