1497-pT7A3-ToughTaq

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Cloning of 1497-pT7A3-ToughTaq

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Purpose: Cloning of 1497-pT7A3-ToughTaq

Date: 2012-12-6----2012-12-11

Notebook:0

Page:

Clone ID:1497

Experimenters: yingying

Results

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subcloning

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 lane 7
 lane 8

 1151 pET30b-sso7d-ToughTaq
 1279 pT7A3
enzymes
   Nde1 + Xho1



Colony Screening

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Date: 2012-12-11

Make miniprep and digest with Nde1 + Xho1

clone 5 right


Result intepretation/Summary

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  • <br>

Conclusion

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<br>


QC

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2013-8-15

15ul elution in 300 mM Imidozale, 20 mM Tris-HCl pH 8.0, 600 mM NaCl

PCR test


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