1826-pCold-GST-(3C)-FluA-NP
Cloning of 1826-pCold-GST-(3C)-FluA-NP
[edit]Purpose: Cloning of 1826-pCold-GST-(3C)-FluA-NP
Date: 2013-8-26---2013-8-27
Notebook:0
Page:
Clone ID:1826
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 1832 FluA-NP as template.
FluA-NP-Nde1-F
FluA-NP-Xho1-R
| lane |
1 | 2 | 3 |
| PCR product |
1629,pCold-GST-(3C) |
1697,pCold-GST-(3c)-wt3C | |
| enzymes |
Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2013-8-27
Make miniprep and digest wit Nde1 + Xho1
no insert
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1826-pCold-GST-(3C)-FluA-NP
[edit]Purpose: Cloning of 1826-pCold-GST-(3C)-FluA-NP
Date: 2013-8-28---2013-8-29
Notebook:0
Page:
Clone ID:1826
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 1832 FluA-NP as template.
FluA-NP-Nde1-F
FluA-NP-Xho1-R
| lane |
1 | 2 | |
| PCR product |
1629,pCold-GST-(3C) |
||
| enzymes |
Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2013-8-29
Make miniprep and digest wit Nde1 + Xho1
no insert
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1826-pCold-GST-(3C)-FluA-NP
[edit]Purpose: Cloning of 1826-pCold-GST-(3C)-FluA-NP
Date: 2013-8-30----
Notebook:0
Page:
Clone ID:1826
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 1832 FluA-NP as template.
FluA-NP-Nde1-F
FluA-NP-Xho1-R
| lane |
1 |
2 |
3 |
4 |
| PCR product |
1454,pEC |
PCR product |
1697,pCold-GST-(3c)-wt3C | |
| enzymes |
Nde1+Xho1 | |||
Colony Screening
[edit]Date: 2013-
Make miniprep and digest
no insert
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1826-pCold-GST-(3C)-FluA-NP
[edit]Purpose: Cloning of 1826-pCold-GST-(3C)-FluA-NP
Date: 2013-9-6------2013-9
Notebook:0
Page:
Clone ID:1826
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane |
1 |
2 | ||
| 1840-3,pT7A3-flu-NP |
1697,pCold-GST-(3c)-wt3C | |||
| enzymes |
Nde1+Xho1 | |||
Colony Screening
[edit]Date: 2013-
Make miniprep and digest
Result intepretation/Summary
[edit]Conclusion
[edit]