664-pEC-Eag1

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Cloning of 664-pEC-Eag1

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Purpose: Cloning of 664-pEC-Eag1

Date: 2013-9-3---2013-9-6

Notebook:0

Page:

Clone ID:664

Experimenters: yingying

Results

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cloning

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pcr1:Ega1-1-1 to Ega1-1-12

pcr2:Ega1-2-1 to Ega1-2-12



PCR: pcr1+pcr2

Ega1-1-1 to Ega1-2-12



lane
 1
 2

 PCR product
1454,pEC
enzymes
      Nde1 + Xho1


Colony Screening

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Date: 2013-9-9

Make miniprep and digest with Nde1 + Xho1


fix clone1

2013-9-13

Nde1 + BamH1




Result intepretation/Summary

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Conclusion

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