1845-pJ912-PKAalpha

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Cloning of 1845-pJ912-PKAalpha

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Purpose: Cloning of 1845-pJ912-PKAalpha

Date: 2013-9-16---2013-9-20

Notebook:0

Page:

Clone ID:1845

Experimenters: yingying

Results

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cloning

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PCR using 1 pSpeedET-PKAalpha as template
pcr1
PKAalpha-6XHis-Xho1-F
PKAalpha-sm-R

pcr2
PKAalpha-sm-F
PKAalpha-Not1-R



PCR again using pc1+pcr2
PKAalpha-6XHis-Xho1-F
PKAalpha-Not1-R


lane
 1 2

PCR product
 1842 pJ912-Irisin-1

 Xho1 + Eag1



Colony Screening

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Date: 2013-9-20

Make miniprep and digest with Xho1 + Eag1




Result intepretation/Summary

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Conclusion

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