1849 1813 1811 1824 1825

Date: 2013-9-20

FN3K and FLP purification

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Clone ID	1849	1849	1813	1813	1813	1813	1813	1811	1811		1824	1825
Name	FN3K	FN3K	HTNFlp-Ecoli	HTNFlp-Ecoli	HTNFlp-Ecoli	HTNFlp-Ecoli	HTNFlp-Ecoli	HTNFlp-Ecoli	pT7A3-VPol1	Fermentas PageRuler Plus	pCold-MBP-(a3C)-NRF2-3C	pCold-MBP-(a3C)-NRF2-pLacZ-3C
Desc	50 mM NaCl, 300 mM Imidazole elute from Ni column	500 mM NaCl, 300 mMImidazole elute from Ni column	Q column flow through	Q column eluted with 1 M NaCl	SP flow through				elute from Ni column		small scall expression screening	small scall expression screening
Sample treatment	load sample to Ni column, wash with Buffer A	load sample to Ni column, wash with Buffer A	concentrate Ni resin purified protein by spin column, buffer exchange to adjust salt conc to 50 mM NaCl, load to Q column	lconcentrate Ni resin purified protein by spin column, buffer exchange to adjust salt conc to 50 mM NaCl, load to Q column	concentrate Ni resin purified protein by spin column, buffer exchange to adjust salt conc to 50 mM NaCl, load to SP column				load sample to Ni column, wash with Buffer A