1836 pCold-MBP-Taq-Muts

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Cloning of 1836 pCold-MBP-Taq-Muts

[edit]

Purpose: Cloning of1836 pCold-MBP-Taq-Muts

Date: 2013-8-30--

Notebook:0

Page:

Clone ID:1836

Experimenters: Shanyi

Results

[edit]

cloning

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PCR from 352 pET3a-Muts

Taq-Muts-F CAT ACA TAT GGA AGG CAT GCT GAA GGG C

Taq-Muts-HindIII-R tgctagAAGCTTCCCCTTCATGGTATCCAAGG


digest with Nde1 + HindIII,


Colony Screening

[edit]

Date: 2013-8-30

Make miniprep and digest with HindIII+BamHI


Cloning of 1836 pCold-MBP-Taq-Muts

[edit]

Purpose: Cloning of1836 pCold-MBP-Taq-Muts

Date: 2013-8-30--

Notebook:0

Page:

Clone ID:1836

Experimenters: Shanyi

Results

[edit]

cloning

[edit]

PCR from 352 pET3a-Muts

Taq-Muts-F CAT ACA TAT GGA AGG CAT GCT GAA GGG C

Taq-Muts-HindIII-R tgctagAAGCTTCCCCTTCATGGTATCCAAGG


digest with Nde1 + HindIII,


Colony Screening

[edit]

Date: 2013-8-30

Make miniprep and digest with


Result intepretation/Summary

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Conclusion

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