1890-pCMV5-MMLV-gag-NoStop-WPRE-oriP

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Cloning of 1890-pCMV5-MMLV-gag-NoStop-WPRE-oriP

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Purpose: Cloning of 1890-pCMV5-MMLV-gag-NoStop-WPRE-oriP

Date: 2013-10-23---2013-10-25

Notebook:0

Page:

Clone ID:1890

Experimenters: yingying

Results

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cloning

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PCR from 1713 MMLV
MMLV-Gag-BamH1-F
MMLV-Gag-EcoR1-R


PCR product
 1449,pCMV5-Gag-NoStop-WPRE-oriP

            BamH1 + EcoR1


Colony Screening

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Date: 2013-10-25

Make miniprep and digest with BamH1 + EcoR1/ BamH1 + sph1

c: 1449pCMV5-Gag-NoStop-WPRE-oriP


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Result intepretation/Summary

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Conclusion

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