1902 pT7A3-6H-sso7d-T7-DNA-Ligase

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Cloning of 1902 pT7A3-6H-sso7d-T7-DNA-Ligase

Purpose: Cloning of 1902 pT7A3-6H-sso7d-T7-DNA-Ligase

Date: 2013-10-22---

Notebook:0

Page:

Clone ID:1902

Experimenters: shanyi

Results

CLONING

subclone T7 DNA ligase from 1464 pT7A3-T7 DNA ligase or 1037 p8H-T7ligase to 1846 pT7A3-6H-sso7d using Nde1 + Xho1

Colony Screening

Date: 2013-10-24

Make miniprep, digest with Nco1 + Nde1 + Xho1--> T4 DNAligase + sso7d bands

stock1902-2

Result intepretation/Summary

Conclusion

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Cloning