1903 pT7A3-6H-sso7d-T3-DNA-Ligase

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Cloning of 1903 pT7A3-6H-sso7d-T3-DNA-Ligase

Purpose: Cloning of 1903 pT7A3-6H-sso7d-T3-DNA-Ligase

Date: 2013-10-22---

Notebook:0

Page:

Clone ID:1903

Experimenters: shanyi

Results

CLONING

subclone T3 DNA ligase from 1096 pET28b-T3-Ligase or 1500 pT7A3-T3-ligase to 1846 pT7A3-6H-sso7d using Nde1 + Xho1

Colony Screening

Date: 2013-10-24

Make miniprep, digest with Nco1 + Nde1 + Xho1--> T3 DNAligase + sso7d bands

stock 1903-3

Result intepretation/Summary

Conclusion

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Cloning