1854-pPinkalpha-HC-yFLP

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Cloning of 1854-pPinkalpha-HC-yFLP

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Purpose: Cloning of 1854-pPinkalpha-HC-yFLP

Date: 2013-10-28---2013-10-31

Notebook:0

Page:

Clone ID:1854

Experimenters: yingying

Results

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cloning

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PCR using 1853 pET28b-yFLP as template

6XHis-Xho1-F2
Flp-Kpn1-R


lane
 1
 2

 PCR product
 215 pPinkalpha-HC

 Xho1 + Kpn1


Colony Screening

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Date: 2013-10-31

Make miniprep and digest with Xho1 + Kpn1/Hind111




Result intepretation/Summary

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Conclusion

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