1875 pFastBac-MMVp-VP-2
Cloning of 1875 pFastBac-MMVp-VP-2
[edit]Purpose: Cloning of 1875 pFastBac-MMVp-VP-2
Date: 2013-11-6---
Notebook:0
Page:
Clone ID:1875
Experimenters: shanyi
Results
[edit]SUBCLONING
[edit]subcloning using Sal1 + Xba1 from 1874 to 49 pFastBac1
Colony Screening
[edit]Date: 2013-11-8
Make miniprep, digest with Sal1 + Xba1
Analyzing Recombinant Bacmid DNA by PCR
Use these two primers to do PCR
M13-F CCCAGTCACGACGTTGTAAAACG
M13-R AGCGGATAACAATTTCACACAGG
PCR product should be ~2300 bp + 1764bp~5000bp. If it is only ~300 bp, then is is empty vector.
