1877 pFastBac-MMV VP2 Strep II
Cloning of 1877 pFastBac-MMV VP2 Strep II
[edit]Purpose: Cloning of 1877 pFastBac-MMV VP2 Strep II
Date: 2013-11-6---
Notebook:0
Page:
Clone ID:1877
Experimenters: shanyi
Results
[edit]SUBCLONING
[edit]subcloning using EcoR1 + HindIII from 1876 to 49 pFastBac1
Colony Screening
[edit]Date: 2013-11-8
Make miniprep, digest with EcoR1 + HindIII
Analyzing Recombinant Bacmid DNA by PCR
Use these two primers to do PCR
M13-F CCCAGTCACGACGTTGTAAAACG
M13-R AGCGGATAACAATTTCACACAGG
PCR product should be~5000bp. If it is only ~300 bp, then is is empty vector.
