1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP

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Cloning of 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP

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Purpose: Cloning of 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP

Date: 2013-12-4---

Notebook:0

Page:

Clone ID:1969

Experimenters:  shanyi

Results

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cloning

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PCR out Gag from 1445 pCMV5-Gag-NLS-Flp-WPRE-oriP using Gag-BamH1-F+HIV-Gag-Kpn1-EcoR1-R


digest with EcoR1 + BamH1 and clone back into 1445 pCMV5-Gag-NLS-Flp-WPRE-oriP

Colony Screening

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Date: 2013-12-5

Make miniprep, digest with EcoR1 + BamH1


send 1969-1 to sequence with HIV-Gag-seq-F




Result intepretation/Summary

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Conclusion

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