622 623 625 654 655 1757 1801 1816 1814 1815

Protein Expression

2013/8/15
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw
1	pT7A3-8H-IL-2	IL-2	622	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTGRT o/n	Ni beads	5.97/18kd
2	pT7A3-8H-IL-4	IL-4	623	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	5.97/18kd
3	pT7A3-8H-IL-15	IL-15	625	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	5.74/19kd
M	PageRuler Plus Prestained protein ladder
4	pT7A3-Monellin-E	Monellin-E	654	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.0/11.4kd
5	pT7A3-Monellin-y	Monellin-y	655	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.68/11.2kd
6	pT7A3-MBP-(3C)-FN3K	FN3K	1757	5 ml, Nico21(DE3)pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	81kd
7	pT7A30-KOD-sso7d	KOD-sso7d	1801-1	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.28/102kd
8	pT7A30-KOD-sso7d	KOD-sso7d	1801-2	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.28/102kd
9	pCold-MBP-(E3C)-Enterokinase	Enterokinase	1816	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	5.90/27kd
10	pMAL-p5E-Enterokinase	Enterokinase	1814	5 ml, BL21DE3pRare2pLysS	0.2mM IPTG RT o/n	Ni beads	5.90/27kd
11	pMAL-c5E-Enterokinase	Enterokinase	1815	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	5.90/27kd