1315-pT7A3-MthRnl
Cloning of 1315-pT7A3-MthRnl
[edit]Purpose: Cloning of 1315-pT7A3-MthRnl
Date: 2013-11-3---2013-11-6
Notebook:0
Page:
Clone ID:1315
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:MthRnl-1-1 to MthRnl-1-16
pcr2:MthRnl-2-1 to MthRnl-2-16
PCR:MthRnl-1-1 +MthRnl-2-16
| lane |
1 |
2 |
| PCR product |
1225,pET28b-Endo1 | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2013-11-6
Make miniprep and digest with Nde1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1315-pT7A3-MthRnl
[edit]Purpose: Cloning of 1315-pT7A3-MthRnl
Date: 2013-12-30---2014-1-2
Notebook:0
Page:
Clone ID:1315
Experimenters: yingying
gene synthesis
pcr1:MthRnl-1-1 to MthRnl-1-16
pcr2:MthRnl-2-1 to MthRnl-2-16
PCR:MthRnl-1-1+ MthRnl-2-16
| lane |
1 |
2 |
| PCR |
1279 pT7A3 | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-1-2
Make miniprep and digest with Nde1 + Xho1
