1961-pCMV5-HTNCre
Cloning of 1961-pCMV5-HTNCre
[edit]Purpose: Cloning of 1961-pCMV5-HTNCre
Date: 2013-12-30---2-14-1-7
Notebook:0
Page:
Clone ID:1961
Experimenters: yingying
Results
[edit]cloning
[edit]
1. PCR His-Tat-NLS from 1708 pT7A3-HTNCre
pcr1
1708 pT7A3-HTNCre
6His-Nhe1-F
NLS-HindIII-R
PCR2 Flpo from 1520 pDIRE
NLS-HindIII-F
iCre-Eag1-Kpn1-R
PCR Then join two fragments
6His-Nhe1-F
iCre-Eag1-Kpn1-R
| lane |
3 |
4 |
| pcr |
1758 pCMV5-hIgG-Lc-FN3K | |
| Nhe1 + Kpn1 |
Colony Screening
[edit]Date: 2014-1-6
Make miniprep and digest with Nhe1 + Kpn1/hind111 + Kpn1
Result intepretation/Summary
[edit]Conclusion
[edit]