1693 pT7A3-8H-LambdaPP
Cloning of 1693 pT7A3-8H-LambdaPP
[edit]Purpose: Cloning of1693 pT7A3-8H-LambdaPP
Date: 2014-1----
Notebook:0
Page:
Clone ID:1693
Experimenters: shanyi
Results
[edit]cloning
[edit]PCR using Lambda phage DNA
LambdaPP-Nde1-F
cgatca catatgcgct attacgaaaa aattg
LambdaPP-Xho1-R
(gt acagggagaa ggcgcatgactcgag ctagca)
tgctagctcgagtcatgcgccttctccctgtac
digest with Nde1 + Xho1, ligate to Nde1 + Xho1 digested 1725 pT7A3-8H
Colony Screening
[edit]Date: 2013-10-23
Make miniprep, digest with Nde1 + Xho1
