1942 pT7A3E-new-phi29
Cloning of 1942 pT7A3E-new-phi29
[edit]Purpose: Cloning of1942 pT7A3E-new-phi29
Date: 2014-1----
Notebook:0
Page:
Clone ID:1942
Experimenters: shanyi
Results
[edit]cloning
[edit]PCR using 1617 pT7A3-MBP-(3c)-newPhi29 as template
New-Phi29-1-1 A1 ATCGACGCTAGCATGAAACATATGCCACGCAAAATG plate 1_10_2013
newPhi29-Sal1-R (GACGATACCTTTACCATTAAGgtcgac tctaca) tgtagagtcgacCTTAATGGTAAAGGTATCGTC
digest vector 1892 pT7A3E with Nde1 + Xho1, Digest PCR product with Nde1 + Sal1
Colony Screening
[edit]Date: 2013-10-23
Make miniprep, digest with NdeI+XHOI
