725-pBad24-sfGFP-T30C-A67G

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Cloning of 725-pBad24-sfGFP-T30C-A67G

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Purpose: Cloning of 725-pBad24-sfGFP-T30C-A67G

Date: 2014---1-16---

Notebook:0

Page:

Clone ID:725

Experimenters: yingying

Results

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cloning

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Cloning Method

PCR using pBad24-sfGFP as template ~ 15 to 20 cycles
new oligo
SfGFP-T30C-A67G-F A12
Ecoli-sfGFP20


gel purify PCR product

then PCR again using gel purified DNA
new oligp
sfGFP-T30C-EcoR1-F
Ecoli-sfGFP20


lane
 1
 2

 pcr
 pBad24 545

 EcoR1 + HindIII


Colony Screening

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Date: 201

Make miniprep and digest with



Result intepretation/Summary

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Conclusion

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