Promoter Leakage

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1/17/2014


724 pBad24-sfGFP, pBad promoter
 2027 pT5-sfGFP, T5 promoter
2042 pT7A3-sfGFP, T7A3 promoter 2043 pL42-sfGFP, lambda promoter


1/18/2014

2026 pT7A3-T4 ligase-HI (no GFP control) 725 pBad24-sfGFP, pBad promoter
2043 pL42-sfGFP, lambda promoter, grow at 30 oc
  724 pBad24-sfGFP, pBad promoter, 0.5% glucose


2043 pL42-sfGFP, lambda promoter

grow at 30 oC

2041 pT7A3E-HC-sfGFP, high copy T7A3 promoter

725 pBad24-sfGFP, pBad promoter

725 pBad24-sfGFP
  724 pBad24-sfGFP, pBad promoter, 0.5% glucose

grow at 30 oc

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