1972-pCMV5-Tat-Cre-PDGFR

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Cloning of 1972-pCMV5-Tat-Cre-PDGFR

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Purpose: Cloning of 1972-pCMV5-Tat-Cre-PDGFR

Date: 2014-1-14---2014-1-23

Notebook:0

Page:

Clone ID:1972

Experimenters: yingying

Results

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cloning

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pcr1 the same with 1961pcr1

pcr2 PCR iCre from 1520 pDIRE
      NLS-iCre-HindIII-F iCre-Kpn1-R


PCR :pcr1+pcr2



lane
3
 4

 pcr
1971 pCMV5-hIgG-Lc-FN3K-PDGFR-WPRE-OriP

 Nhe1 + Kpn1


Colony Screening

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Date: 204-1-23

Make miniprep and digest with Hind111 + Kpn1



Result intepretation/Summary

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Conclusion

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